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Puttubayh:
Puttubayh is the incarge of the Corporation that's ship was hijacked. He was attempting to carry Zeiram, and invincible space monster, to a lab to make him a bio weapon. During the hijacking, the hijackers must've thought the cargo was something else. When thet freed Zeiram, he killed the hijackers and took control of the ship and began to attack corporation workers. Puttabayh gave the workers who survived, new identities and made Bob a computer for his own use. When he learned Iria had survived and had the only proof of his scheme, he sent out to kill her. Instead, she stole Bob, kept the evidence and destroyed the inside of his corporation buiding.

Dr. Touka:
Dr. Touka is the only Zeiram expert left. Years before the Puttubayh incodent, when Zeiram was first rumored about, he had been apart of a team that studied Zeiram. When they discovered how dangerous it was, the program was shut down by the government and no one was allowed to even attempt to capture Zeiram for any purpose. He was sent to Myce when Zeiram showed up. On board, Kei adn Komimasa hid. The doc's ship was hijacked and Zeiram clones killed the hijackers once on Myce. Iria showed up to help the doc and ran into a clone of Gren among the Zeirams. She fought with the evil Gren and was hesitant because it looked just like her brother. When the evil clone killed Komimasa, Iria got pissed and killed the clone. After this happened, Dr. Touka collected samples to study Zeiram and was able to predict a Zeiram attacked. The administrators laughed at his ideas to destroy Zeiram. Back in his lab, he ran into Iria and Kei who had listened to the conversation. They had a plan to get Zeiram and the doc told them everything they needed to know.

Zeiram:
Zeiram is an unkillable, evil space creature captured by Puttubayh to be used as a bio a weapon. When it was released by accident, it ran wild with one thing on its mind; kill anything that moves. When the hijacked ship, the Karma, was nuked by Iria and Gren, Zeiram was barely alive. He somehow found the alive Gren and fed off his life support and took over gren to make himself a new body, leaving the helpless Gren to watch in horro as hi body was used to attack people, even his own sister. Gren breaks free of Zeiram for a few seconds, and tells Iria he's sorry and to destroy Zeiram by killing him. If you look closely while watching the animation, after the Karma incident, Zeiram is wearing Gren's armor in the Shadow Zone and you can make out a red eye on his neck.

<---- this is what gren looks like when he free's himself from Zeiram for a few seconds to talk to Iria
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